Coding

Part:BBa_K1457000:Design

Designed by: Michelle Long, Nicholas Larus-Stone, Jonah Saltzman, Tiana Raphel   Group: iGEM14_Harvard_BioDesign   (2014-10-09)


mRFP_Spycatcher


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is a direct fusion of mRFP and the Spycatcher sequence. We would have liked to include a linker between the two sequences in the original design.


Source

mRFP from team Uppsala-Sweden 2011: BBa_E1010 Spycatcher from addgene, Howarth lab: CnaB2114

References